Effect of acemannan on the dentinsialophosphoprotein and dentin matrix protein 1 mRNA expressions in primary human pulpal cells

Authors :

• Pechanika Lardungdee D.D.S., M.S.
• Pornpun Asvanit D.D.S., M.S.
• Pasutha Thunyakitpisal D.D.S., Ph.D.

Abstract :

Objective To investigate the effect of acemannan isolated from Aloe vera gel on the steady level of
dentinsialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) mRNAs in primary human
pulpal cells.

Materials and methods Cells were treated with the designated concentrations of acemannan (0.25,
0.5 and 1 mg/ml) for 24 hours. The reverse transcription-polymerase chain reaction assays were used
to investigate the effects of acemannan on the steady level of DSPP and DMP 1 mRNAs.

Results At 24 hours of incubation, acemannan (0.5 mg/ml) significantly enhanced the expressions of
DSPP and DMP 1 mRNA levels up to 1.93 and 2.76 fold, respectively, as compared with the control
group (p < 0.05).

Conclusion Acemannan at concentration 0.5 mg/ml stimulated both DSPP and DMP 1 mRNA expressions
in human dental pulp cells.

(CU Dent J. 2008;31:407-14)

Keywords :
acemannan; Aloe vera; dental pulp cell; dentin matrix protein 1; dentinsialophosphoprotein